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construct encoding egfp nanos3  (New England Biolabs)


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    New England Biolabs construct encoding egfp nanos3
    A) Schematic of the experimental strategy. eGFP-nanos3’UTR containing mRNA was injected into 1-cell stage zebrafish embryos from which two different experimental approaches were used to investigate the ratio of maternal and somatic ribosomes in PGCs and to test which ribosomes are bound to PGC-localized mRNAs. B) Brightfield and eGFP images of 1 day post-fertilization embryos, previously injected with only water, eGFP-nanos3’UTR mRNA, or biotinylated <t>eGFP-nanos3-3’UTR</t> mRNA. Scale bars indicate 500 μm. C) Ratio of maternal (yellow) versus somatic (blue) 18S and 28S rRNA in FACS-isolated eGFP-positive (PGCs) and eGFP-negative (somatic) cells analyzed by RT-qPCR. D) Ratio of maternal (yellow) versus somatic (blue) 18S and 28S variants associated with biotinylated eGFP-nanos3-3’UTR mRNAs isolated by RIP (RNA immunoprecipitation). Non-biotinylated eGFP-nanos3-3’UTR mRNA served as control. Non-specific (background) amplification was determined using IPs from wild-type (uninjected) lysate and was set to zero. In (C) and (D), three biological replicates from independent natural crosses were used, and significance was calculated by t-test (if not indicated, p-value > 0.05).
    Construct Encoding Egfp Nanos3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1097 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/construct encoding egfp nanos3/product/New England Biolabs
    Average 97 stars, based on 1097 article reviews
    construct encoding egfp nanos3 - by Bioz Stars, 2026-05
    97/100 stars

    Images

    1) Product Images from "A dual ribosomal system in the zebrafish soma and germline"

    Article Title: A dual ribosomal system in the zebrafish soma and germline

    Journal: bioRxiv

    doi: 10.1101/2024.08.29.610041

    A) Schematic of the experimental strategy. eGFP-nanos3’UTR containing mRNA was injected into 1-cell stage zebrafish embryos from which two different experimental approaches were used to investigate the ratio of maternal and somatic ribosomes in PGCs and to test which ribosomes are bound to PGC-localized mRNAs. B) Brightfield and eGFP images of 1 day post-fertilization embryos, previously injected with only water, eGFP-nanos3’UTR mRNA, or biotinylated eGFP-nanos3-3’UTR mRNA. Scale bars indicate 500 μm. C) Ratio of maternal (yellow) versus somatic (blue) 18S and 28S rRNA in FACS-isolated eGFP-positive (PGCs) and eGFP-negative (somatic) cells analyzed by RT-qPCR. D) Ratio of maternal (yellow) versus somatic (blue) 18S and 28S variants associated with biotinylated eGFP-nanos3-3’UTR mRNAs isolated by RIP (RNA immunoprecipitation). Non-biotinylated eGFP-nanos3-3’UTR mRNA served as control. Non-specific (background) amplification was determined using IPs from wild-type (uninjected) lysate and was set to zero. In (C) and (D), three biological replicates from independent natural crosses were used, and significance was calculated by t-test (if not indicated, p-value > 0.05).
    Figure Legend Snippet: A) Schematic of the experimental strategy. eGFP-nanos3’UTR containing mRNA was injected into 1-cell stage zebrafish embryos from which two different experimental approaches were used to investigate the ratio of maternal and somatic ribosomes in PGCs and to test which ribosomes are bound to PGC-localized mRNAs. B) Brightfield and eGFP images of 1 day post-fertilization embryos, previously injected with only water, eGFP-nanos3’UTR mRNA, or biotinylated eGFP-nanos3-3’UTR mRNA. Scale bars indicate 500 μm. C) Ratio of maternal (yellow) versus somatic (blue) 18S and 28S rRNA in FACS-isolated eGFP-positive (PGCs) and eGFP-negative (somatic) cells analyzed by RT-qPCR. D) Ratio of maternal (yellow) versus somatic (blue) 18S and 28S variants associated with biotinylated eGFP-nanos3-3’UTR mRNAs isolated by RIP (RNA immunoprecipitation). Non-biotinylated eGFP-nanos3-3’UTR mRNA served as control. Non-specific (background) amplification was determined using IPs from wild-type (uninjected) lysate and was set to zero. In (C) and (D), three biological replicates from independent natural crosses were used, and significance was calculated by t-test (if not indicated, p-value > 0.05).

    Techniques Used: Injection, Isolation, Quantitative RT-PCR, RNA Immunoprecipitation, Control, Amplification



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    construct encoding egfp nanos3  (New England Biolabs)
    97
    New England Biolabs construct encoding egfp nanos3
    A) Schematic of the experimental strategy. eGFP-nanos3’UTR containing mRNA was injected into 1-cell stage zebrafish embryos from which two different experimental approaches were used to investigate the ratio of maternal and somatic ribosomes in PGCs and to test which ribosomes are bound to PGC-localized mRNAs. B) Brightfield and eGFP images of 1 day post-fertilization embryos, previously injected with only water, eGFP-nanos3’UTR mRNA, or biotinylated <t>eGFP-nanos3-3’UTR</t> mRNA. Scale bars indicate 500 μm. C) Ratio of maternal (yellow) versus somatic (blue) 18S and 28S rRNA in FACS-isolated eGFP-positive (PGCs) and eGFP-negative (somatic) cells analyzed by RT-qPCR. D) Ratio of maternal (yellow) versus somatic (blue) 18S and 28S variants associated with biotinylated eGFP-nanos3-3’UTR mRNAs isolated by RIP (RNA immunoprecipitation). Non-biotinylated eGFP-nanos3-3’UTR mRNA served as control. Non-specific (background) amplification was determined using IPs from wild-type (uninjected) lysate and was set to zero. In (C) and (D), three biological replicates from independent natural crosses were used, and significance was calculated by t-test (if not indicated, p-value > 0.05).
    Construct Encoding Egfp Nanos3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/construct encoding egfp nanos3/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    construct encoding egfp nanos3 - by Bioz Stars, 2026-05
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    A) Schematic of the experimental strategy. eGFP-nanos3’UTR containing mRNA was injected into 1-cell stage zebrafish embryos from which two different experimental approaches were used to investigate the ratio of maternal and somatic ribosomes in PGCs and to test which ribosomes are bound to PGC-localized mRNAs. B) Brightfield and eGFP images of 1 day post-fertilization embryos, previously injected with only water, eGFP-nanos3’UTR mRNA, or biotinylated eGFP-nanos3-3’UTR mRNA. Scale bars indicate 500 μm. C) Ratio of maternal (yellow) versus somatic (blue) 18S and 28S rRNA in FACS-isolated eGFP-positive (PGCs) and eGFP-negative (somatic) cells analyzed by RT-qPCR. D) Ratio of maternal (yellow) versus somatic (blue) 18S and 28S variants associated with biotinylated eGFP-nanos3-3’UTR mRNAs isolated by RIP (RNA immunoprecipitation). Non-biotinylated eGFP-nanos3-3’UTR mRNA served as control. Non-specific (background) amplification was determined using IPs from wild-type (uninjected) lysate and was set to zero. In (C) and (D), three biological replicates from independent natural crosses were used, and significance was calculated by t-test (if not indicated, p-value > 0.05).

    Journal: bioRxiv

    Article Title: A dual ribosomal system in the zebrafish soma and germline

    doi: 10.1101/2024.08.29.610041

    Figure Lengend Snippet: A) Schematic of the experimental strategy. eGFP-nanos3’UTR containing mRNA was injected into 1-cell stage zebrafish embryos from which two different experimental approaches were used to investigate the ratio of maternal and somatic ribosomes in PGCs and to test which ribosomes are bound to PGC-localized mRNAs. B) Brightfield and eGFP images of 1 day post-fertilization embryos, previously injected with only water, eGFP-nanos3’UTR mRNA, or biotinylated eGFP-nanos3-3’UTR mRNA. Scale bars indicate 500 μm. C) Ratio of maternal (yellow) versus somatic (blue) 18S and 28S rRNA in FACS-isolated eGFP-positive (PGCs) and eGFP-negative (somatic) cells analyzed by RT-qPCR. D) Ratio of maternal (yellow) versus somatic (blue) 18S and 28S variants associated with biotinylated eGFP-nanos3-3’UTR mRNAs isolated by RIP (RNA immunoprecipitation). Non-biotinylated eGFP-nanos3-3’UTR mRNA served as control. Non-specific (background) amplification was determined using IPs from wild-type (uninjected) lysate and was set to zero. In (C) and (D), three biological replicates from independent natural crosses were used, and significance was calculated by t-test (if not indicated, p-value > 0.05).

    Article Snippet: To generate the RNA for microinjections, 2 μg of a construct encoding eGFP-nanos3’UTR was digested using NotI-HF (New England BioLabs, R3189S) for 1 h. The linearized product was purified according to manufacturer specifications via gel selection using NucleoSpin (Macherey-Nagel, 740588.50).

    Techniques: Injection, Isolation, Quantitative RT-PCR, RNA Immunoprecipitation, Control, Amplification